Phylogenetic Relationship of some Indian Chironomids based on mitochondrial DNA Cytochrome Oxidase I
نویسندگان
چکیده
In this study, two individuals of Tanypus chinesis, Chironomus kiiensis, Polypedilium nubifer and one individual of procladius denticulatus, Glyptotendipes meridionalis, Clinotanypus sp, Arielulus circumdatus were collected from the aquatic ecosystem of varthur lake, Bangalore, India. The mitochondrial DNA has been successfully isolated using modified CTAB method and 650 bp fragment of mitochondrial gene cytochrome oxidase subunit I (COI) has been amplified and sequenced. A phylogenetic relationship among these species has been investigated using MEGA 6 software. Sanger sequence divergence was observed between two individuals of the Tanypus chinensis and it was suggested that might be more than one species was involved. However the intraspecific sequence divergence was lower between Inter species divergence. Interestingly, Arielulus circumdatus showed amazing phylogentic relation to Tanypus chinesis and that presumably reflect co-evolutionary traits of different subfamilies. The sequence of the mtDNA cytochrome oxidase subunit I gene has proven useful to investigate the phylogenetic relationship among the ambiguous species of chironomids.
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